Storing Peptides | Learn How to Properly Mix, Dose & Store Peptides

Storing Your Peptides

Storing your peptides correctly whether they are reconstituted or not is pivotal in how they perform. A big difference is in the temperature of when they are reconstituted versus when they are still in lyophilized form.

Typically, when in lyophilized form or reconstituted form the rule of thumb is to maintain a temperature between 36 and 46 degrees Fahrenheit. For those of you that use Celsius that would mean about 2 degrees to 8 degrees. In many cases, this would mean storing it in your freezer which is a perfectly viable option.

Peptide Reconstitution

Proper reconstitution and maintenance of your peptides are pivotal in how well they work for you. If not stored or reconstituted correctly, you risk damage to or degradation of your peptides. But why do you need to reconstitute your peptides in the first place?

These substances mainly come as a compound that has been lyophilized. This phrase basically means that they have been turned into a freeze-dried crystalline powder. In order to use your peptides, you’ll need to add fluid to them. This is where the phrase reconstitution is coined.

However, you cannot add just any old fluid to your compound, and it cannot be done in a listless manner. Great care needs to be taken to ensure that your peptide is not damaged during this process.

How to Reconstitute Peptides

For starters, you’ll need the following supplies for proper peptide reconstitution:

    • An Alcohol Wipe
    • A syringe
    • Your Lyophilized Peptide
    • Bacteriostatic Water or Sterile Water

Stack of Alcohol Wipes To begin the peptide reconstitution process, you’ll want to wipe down the top of your vials as a safety precaution with the alcohol wipe. Then you’ll want to use your syringe and pull either your bacteriostatic or sterile water from that vial. Keep in mind you don’t want to touch the needle as this part of the syringe has been sterilized. Therefore, touching it would then cause contamination.
For the sake of this example, we’ll say that you pull 1ml. Keep in mind, different doses need different amounts of liquid when reconstituting. This will be explained further in the dosing section of this article.
From here, you’ll want to inject your syringe directly into the lyophilized peptide vial. Do not, I repeat, DO NOT just shoot the water into the vial. This will damage the peptide and render it useless.

Johannah Gregg, Certified Family Nurse Practitioner and Peptide Therapy Specialist says, “Imagine shooting a hose of hot water at frozen icicles hanging off your roof. When the hot water makes contact with the icicles, they shatter. You’ll be causing the same shock to the peptide chains, resulting in breakdown of the peptides.”

Rather than spraying your peptide, the goal here is to drip the liquid down the side of the vial. Doing so will begin the process of reconstitution without destroying your product. Once you inject either your bacteriostatic water solution or sterile water into your peptide vial, you can remove the syringe from the vial. Some individuals may say you need to lightly swirl the solution in the vial. However, 9 times out of 10 this is unnecessary and can do more harm than good. You’ll basically want to let it dissolve on its own.

Bacteriostatic Water vs. Sterile Water

During peptide reconstitution, two variations of solutions are popular. These two solutions are sterile water and bacteriostatic water. The choice is purely up to the user which solution to use. However, there are some differences between the two.

Differences Between Bacteriostatic Water vs. Sterile Water

Bottle of Bacteriostatic Water For bacteriostatic water, for instance, it is not like the sterile water in that it can be used several times within a 28-hour span. In fact, because of this reason, bacteriostatic water is fairly popular among scientists. It is considered a non-pyrogenic solution, meaning that it does not conduct heat or fever when introduced to the body. It contains roughly 0.9% benzyl alcohol. This variation of alcohol is an aromatic alcohol. In terms of chemistry, they are a compound that contains a hydroxyl group that is indirectly bonded to an aromatic compound. This gives it a pleasant smell. All of this basically comes down to is that the liquid is made in a way where bacteria cannot grow. That’s what makes it more user friendly since it can be used multiple times.

As for regular sterile water, it too has its own positives. It too is a nonpyrogenic liquid. However, unlike the bacteriostatic water, the sterile water can only be used once and once opened and used would need to be discarded. In many cases, when the body doesn’t particularly fair well with bacteriostatic water, this is a good substitute.

 

Understanding Peptide Dosages

For the purpose of explaining how exactly we get the correct dosage for each peptide, we’ll say we want to go with a experimental treatment regimen of PT 141 also know as Bremelanotide. Now, for PT 141, the vials come as 10mg vials. This means that we not only need to know how to properly reconstitute a peptide, we also need to know how much of the reconstituted peptide we need to pull into the syringe to administer in our tests.

For starters, one insulin syringe is exactly 1mL of fluid. The measurements on the side of the syringe go up by 10s with each tick mark in between being 2 units each. Now, I mentioned that the vials come as 10mg vials. Say you add 1mL of bacteriostatic water to this compound following the instructions above and want a test dosage of 1.75mg. You now have one mL of bacteriostatic water and 10mg of peptide combined to create your reconstituted peptide. Now what?

Conversions When Dosing Peptides

Well, now we need to understand how much we need to pull into our syringe to get the correct dose each time we administer the peptide. How do we do this? Well, there is a little understanding of the old school conversions that need to take place. To start, every 1mg converts to 1000mcg. This would mean that in 1.75mg there is 1750mcg. Now, looking at your syringe, every tick mark converts to being 200mcg. Therefore, in order to get the correct dose of PT 141, you would need to pull up to 9 tick marks of fluid into your vial. This translates into 1800mcg which in turn would mean 1.80mg.

No, this is not right on the money at 1.75mg but you are right there. Now, if you’d rather be safe and stay under, you’d only need to figure out the next closest dose which on the syringe would end up being 1600mcg or 1.60mg.
Now let’s say you want to do the opposite and up your dose to 2mg per dose. This would then convert to 2000mcg. Again, each tick mark is 200mcg so this would mean that you would need to pull in 10 tick marks. On the syringe, this coincides with the 20 units mark.

Let’s say you end up putting 2mL of bacteriostatic water instead of the 1mL. This effectively makes each tick worth 100mcg. So now if you still want your 2mg of peptide, you’ll have to pull up to the twentieth tick mark which then coincides with the 40 units mark instead of the 20 unit mark.

So, in reality, it will depend on both the number of peptides you have in mg as well as the amount of bacteriostatic or sterile water you put in your peptide vial.

So now say we have a peptide that has 5mg of product in the vial and you reconstitute it with 1mL of bacteriostatic water. This ends up being the same as having 10mg of product and 2mL of bacteriostatic water. In that in order to get your 2mg dose, you’ll need to hit that 40 units tick mark because each tick is worth 100mcg.

Learn More About Peptides

Discover all there is to know about peptides and proper care for them. The experts at Paradigm Peptides have years of experience in the industry and can help you every step of the way. Whether you have questions about different types of peptides, procedures, storage, or anything else, feel free to read our peptide therapy guide today and contact our team with any questions. Visit our online store to browse our USA-made peptides for sale.

107 thoughts on “How to Properly Mix, Measure, and Store Peptides”

  1. 10mg bottle of tb500, going with 200mcg two times a day, twice a week for tendon repair post surgery. how much bacteriostaric water do i add and then how much do should go in the needle prior to injection

      • This maybe a little late in putting in this info. I gave a lot of shots as an RN. Buy some insulin syringes with a 30 gauge needle. That gauge of needle slides into the skin with no pain unless you hit a nerve. And even then it is not that bad. Make sure the alcohol is dry otherwise the injection will burn. Next. use water in a syringe and practice on an orange. The skin is tougher but the concept is there. Or peel the orange and inject the water directly into the fruit. After a few times you will develop confidence. Remember when you inject into the abd with a short needle you will probably bruise and you could end up with bruises all over the belly. If you use the abd you need a short needle about 1/4 in. If you go into the thigh a 1″ or 1/2″ needle is what you want to use.

        • I have a 20mg vial of Bremelanotide and I need to Reconstitute With BACTERIOSTATIC water. It gave no instructions on how much water to add with peptide. Help please.

    • The Bacteriostatic water and the peptide to be reconstituted should be at room temperature. Any unused portions of either should then be refrigerated.

    • I have a 5 mg vial of reconstituted sermorelin. I used a 3 ml syringe I usually use for my Intramuscular shots. I think I made a mistake by adding water and almost filled up the vial about 90 percent of water. Did I do this right ..How much do I inject

      • I need to know this too! When i went to the clinic, i think they gave me a 9mg vial and put 5ml of water in it.
        So, in my 5mg vial ,i put 2.5ml of water.? I think i should have done 3

  2. hi i’m looking to see if you have any ideas on what i can stack with the igf-1 for growth and weight loos thank you

  3. after reconstituting 50mg of n-acetyl epitalon amidate with bacteriostatic water my mixture is milky and a white substance settles at the bottom after a few minutes? Ive not experienced this before… should i be concerned? should I inject this or throw away??? advise

    • The N-A version is slightly hydrophobic at its core meaning it will have a look of cloudiness after reconstitution. This is normal and can be seen to an even greater degree in other peptides such as Frag 176-191.

    • Always want it to be clear. Cloudy means it is bad. That is my experience with years of HGH use. Always squirt the water Down the side of the bottle, don’t spray it into the freeze dried crystals.

    • Did you ever get an answer to this question? From what I understand (correct me if you have better information). You would insert 2 1/2 ML of bac water and inject .5 units a day or if the vial is large enough 5ML and inject 10 units on a short 1/4” insulin syringe

  4. I’m a little confused. How much bacteriostatic water should be used when reconstituting 5/5mg CJC 1295/Impamorelin? I’ve read it should be 0.5ml per 1mg. In this case, I’d think I add 5ml to a 5mg/5mg stack. Correct?

    • I use this peptide combination and to a 10 mg blend (5mg cjc/5mg ipamorelin) I add 2 ml bacteriostatic water. Inject 5-10 units (cc) on an insulin syringe for 5 days then skip two days. I administer Monday-Friday then take the weekend off. Should also be administered at least an hour after eating or 20 minutes prior.

      • Thank you for this. I am using the same 10mg blend and wasn’t sure how much Bacteriostatic Water to add.

        Does this last you a full month? I will also be using 10units 5 days a week.

  5. I am a 155# female. I have ordered Tesamorelin 5mg along with Ipamorelin 5mg for weight loss especially in abdominal area. How much should I take of each if I reconstitute with 1mL bac water? I need to know how much I will be injecting nightly for the 6 days/wk so I will know how long it will last for re-ordering. Thanks for any recommendations!

  6. I’m storing my not-yet-reconstituted peptides in the refrigerator, is that ok? I saw a comment earlier that you said to have them at room temperature? Also is frag or tesamorelin better for cutting some body fat?

  7. a little confused. How much bacteriostatic water should be used when reconstituting 5/5mg CJC 1295/Impamorelin? Help with dose too

    • Hello, If you mix 2ml of diluent into the cjc/ipa 5/5 vial that would give you 250 mcg at the 10 mark of your insulin syringe. To get to 300 mcg draw your syringe to the 11.5 mark. I hope this helps. At 250 mcg daily that would give you 20 days worth of product and if you use 300 mcg daily that would give you 16 days of product.

      • What about if the dosage is to be 100 mcg of each? Still 2 ML of diluent but what mark on the insulin syringe?

      • I see that a recommended amount of Ipamorelin is 300mcgs by itsself, Since this is a Blend with CJC1295, wouldnt that 300mcg daily youre referring to actually only be 150mcgs bc its mixed 5/5 with Ipamorelin? Thx

      • Since most websites say that peptides only last “several weeks”, how would this be stored (at what temp? freezer or fridge) if this vial were to be 20/16 days worth of product? Thank You

        • Reconstituted should always be stored in the fridge when not using. Also, storing unmixed in the fridge and out of sunlight preserves it longer

    • bpc-157 comes in different mg strengths. For 10 mg use 6.0 ml water.
      Dosing depends on mcg per shot. 300 mcg=.18ml (18 units on insulin syringe) 333 mcg=.20 ml (20 units), 400 mcg=.24ml (24 units), 500 mcg=.30 (30 units)

      • How do you get 6 ml into a vial that only holds 4 ml at most? Have 10 mg BPC157 and need to know how much draw for 400 mcg dosage?

  8. Hej! can i take igf lr-3 same time as follistatin and how big dose of bouth?
    For how long time can i take them?

  9. I am going to purchase the Epitalon. Please explain the correct mixture of bac water and the dosage on the syringe. How many days to take it and how many days to be off of it
    Thank you.

    • I did 3ML of BW with the 5/5 ipamorelin cjc 1295 mix. Pull to the 10 mark on the insulin syringe will give you 333mcg total (167mcg of each) per day. Should last 30 days.

  10. Hi Paradigm peptides. I’m doing ghrp-6/ghrp-2/cjc 1295 w/o DAC. Could I mix these, reconstituted, in the same vial or would it be wise to keep them separated? It would really simplify extraction but there seems to be different ideas on whether this would break down the peptides or not.

    • Hello, yes these can be mixed together in the same vial after reconstitution. I would suggest taking care when transferring the solution. Reconstituted Peptides are fragile and can deteriorate quickly from moderate shock, gentle swirling or rolling the vials is okay but a drop to a hard surface would render the peptide useless.

  11. I recently purchased epitalon 99% 100milligrams and
    Thymalin 98.46% 100 milligrams
    Suggestions on reconstitution and doses and type of injection.

  12. Need to reconstitute 5mg vial of frag 176-191, but not sure how much bac water to add. Could also use help with dosage please.

  13. Hi Paradigm, The posts are most confusing. Lets establish a constant on info. Tell me where I’m incorrect please. I bought Ipamorelin,CJC1295 5mg. This would be 10mg of powder. The syringe is an insulin syringe U-100. That means 1ml total for the syringe and the major units marked on the syringe 30,40…should represent 300mcg,400 mcgs respectively. The recommended dosage of this Blend is 300 mcgs. This is were it gets confusing for me. The adding of sterile water or BW. If you are adding more BW, like 2ml vs 1ml, then you are “thinning out” the mix. Which would mean you need to pull more (2x) to get the same amount (dosage) of Ipamorelin,CJC1295, correct? So the question to me is, what was that 300mcg recommendation based on 1ml, or 2ml? Please clarify for me and others, the unit pull for 1ml, 2ml adding of BW. My understanding is adding 1ml to the Blend, then pulling to the 30 mark (300mcgs) on the U-100 syringe, would get 16.66 uses out of the vial (300mcg x 16.66=5000mcg [or 5 mg]). Correct? If adding 2ml to the 5/5 Blend, then the 60 mark (600mcg) would be needed bc its peptides are diluted more. Correct?

    • If one syringe equals 1ml how would you possibly get 16 pulls of 30 units off 1ml. You would get 3 pulls and change.

  14. I just bought my first HCG. How much of the bio water that came
    with it do I mix with the HCG and then how often and how much do I inject?

  15. The hcg already comes with sterile water. Its equal to 2mL. Use the whole amount as required to reconstitute hcg powder. and when injecting sterile water into vial always pull air out as well to equal out pressure into the vial.

  16. I bought the GHK-CU Copper Peptide Serum | 50MG. How do I reconstitute it and the dosage? Anyone know?

    • Add 2ml bacteriostatic water, gently of course. Let dissolve do not mix shake swirl. I suggest wait couple hours in fridge. Draw up one MG into syringe. That’d be 2 units. 100 units to a syringe of course. Dosage is 1mg daily.

  17. I got totally confused by this post. I reconstituted frag with 2ML. How many IUs for a 250mcg dose?

  18. After PT-141 has been reconstituted and stored in a fridg, how long does it remain viable? Does it take weeks or months to deteriorate to the point that it is no longer effective?

  19. Hello, Tesamorelin 6mg, CJC1295 3mg, Ipamorelin 3mg, (12mg Blend) Can someone help me on how much Bacteriostatic Water to mix in this blend? and what would be the right doses? and thinner syringe’s, please help!

    • I think so. They say repeatedly not to shake the vial or it renders the peptides useless….

  20. Ipamorelin 5mg and bacteriostatic water 10ml. How much for mixing and how much to pull into the syringe. I have the 1ml syringes

  21. Hi I got Ipamorelin 5mg and 10ml water, and 1ml syringes. I want to start injecting 300mcg of ipamorelin as I am a new user.
    Question how much water I add and how much I should pull into the syringe to gett he correct dosage. Thanks.

  22. Por favor, compre un GHK-CU de 50 mg. Como lo reconstituyo y la dosis de administración

    gracias

  23. Hello,

    I have read all the posts and responses and am very confused.

    I have Tesamorelin 5mg and would like to do a daily injection of 2mg SubQ for 5 days on and 2 days off. How much BW should be mixed during reconstitution and how much should I pull the syringe if I am using a U-100?

    I also have IGF-LR3 0.1mg (100mcg) which I would like to do 30mcg of SubQ for 5 days on and 2 days off. How much BW should be mixed during reconstitution and how much should I pull the syringe if I am using a U-100? Also, how many days can a reconstituted vial stay active if stored in the refrigerator?

    Please advise. Thanks!

  24. First time using blend Sermorelin, GHRP-2 and GHRP-6 BLEND (2mg each = 6mg) and will mix with Bacteriostatic. Advice on missing and dosage 200lbs weight loss lean muscle mass

  25. hello I wanted to know I have CJC-1295/Ipamorelin/GhRP-2 3/3/3MG how much bacteriostatic water can I use
    and can I Take AOD 9604 along with that.

  26. Google peptide calculator. It will give you your **Blanks** to fill in. Like I have 30mg and I want my dose to be 1mg per day. Once you see it everything will make sense.

  27. After you reconstitute BPC-157, how long is it good for? Also, I understand the dosage for weight, but how many doses per day/week? Thank you for your help, plan on ordering once I figure this all out!

  28. Once I receive my BPC-157 and/or TB500 should I reconstitute it right away and put it in the fridge or, dry store it in the fridge and wait until I use it to reconstitute?
    Once reconstituted how long is the shelf life for both of these, of course in the fridge?

    Thanks!
    John D

  29. Good day, kind of needle do I use to take the reconstituted water and mix in with the peptide? Can that same needle then be used to inject the final solution into the body?

  30. Hello, I have BPC-157 & TB-500 Blend 10mg. How much BW should I inject. Also, how much to pull with the U-100 syringe. And how many times a week. Thanks

  31. If I am injecting subcutaneously into abdomen, will the peptide still help with knee and elbow injury?

  32. I could use some help.
    I am starting Ipemorline/CJC 5/5mg

    My does is 10 units on a 1ml/cc insulin’s syringe, Mon-Friday.

    How much Bacteriostatic Water do I need to add to the vile? Is it 2ml?

    Thank you 🙏🏻

  33. I have a few questions I need help with regarding starting CJC1295/Ipemorline 5mg/mg. If taking 300mcg before bed Monday-Friday, how long should that cycle last? I’ve read anywhere from 12 weeks to 6 months so seeking clarity. Also how long of a break is needed between cycles?
    Lastly, are you able to do CJC1295/Ipemorline at the same time as once weekly Tirzepatide? Thanks!

  34. It is astounding and downright scary the number of people here who have not a clue what the heck they are doing. You have no damn business buying these products when you have no idea of the basics of peptide use and the most basic of these is reconstitution and dosage calculation. Please use Google and do some research or hopefully, someone saves you from yourself.

  35. Thanks for the info mate. Quick question if you don’t mind, there is BCP-157 in tablet form, I assume it isn’t as good but I was wondering if you had an opinion for if it is not good at all or if it is just not as good as the injection? It is very hard to get in my country and even speaking about peptides to dr’s has them dismissing me. I have shoulder, hip, knee and back problems that this could really help, but I am stuck.

    • BPC 157 in tablet form will not move out of the gastrointestinal system. BPC 157 taken via Nasal administration will not be absorbed through the sinuses. BPC 157 is only effective with injection close to the damaged tissue as it has poor extracellular matrix permeability.

      • damn. Ok thank you for saving me money 😀 I assume you cannot post to Australia as it needs to be temp controlled?

  36. Fragment, CJC1295, Ipamorelin 12mg how much BW to reconstitute and what’s the best dosage? Thanks for any help up front!

  37. How do you pull air out of a vile with water pulled up. Is that a separate stick? Stick in vile pull back 1ml of air then pull the water 1 ml.

    • Best to use either 3ml or 5ml syringe to mix. Draw up 1ml of air and in the same syringe pull up 1ml of BW. While holding the plunger of the syringe, gently push the air into the air space of your peptide bottle then SLOWLY drip in the 1ml of BW to reconstitute the peptide. Your vial will be pressurized. You would have to do the air into air every time to keep it pressurized. TBH I wouldn’t worry about pressurizing the peptide vial since it’s a multi use vial. I would just draw up the 1ml of BW and slowly drip into the peptide vial.

  38. I have a 10 ml vial of terzepatide ready to be reconstituted. My doctor gave me 2.5 semaglutide. It didn’t do anything for me except cost me a fortune. I have exhausted this dose of 2.5 and I’m ready to move up to 5.0. My question is, how much bacteriostatic water do I mix with 10 mL for a 5.0 dose?

  39. Hi ,

    I purchased
    Fragment 176-191 & Mod GRF 1-29 & Ipamorelin Blend (12mg). Does any know injections daily protocol/ dosage for best weight loss/lean muscle results ? And how much bacteriostatic water do I mix
    With it?

  40. Want to try bpc-157 on my dog for arthritis. Never tried injecting and no nothing of reconstituting. How much bac/sterile water for 5mg vial.

  41. How many doses are in a 5mg once bacterial water is added and how long is it good for in the fridg? How do you know how much to administer each time? How often? Thanks

  42. Can bremelanotide PT141 be intramuscular injection for less holdover time and faster acting. My time of activity was 12 hours.

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